During a stopover in Germany in the middle of a carefree road trip through Europe, two American girls find themselves alone at night when their car breaks down in the woods. Searching for help at a nearby villa, they are wooed into the clutches of a deranged retired surgeon who explains his mad scientific vision to his captives' utter horror. They are to be the subjects of his sick lifetime fantasy: to be the first to connect people, one to the next, and in doing so bring to life "the human centipede."
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| Directing | Tom Six | Director |
| Writing | Tom Six | Writer |
| Sound | Holeg Spies | Music |
| Editing | Nigel de Hond | Editor |
| Art | Stefan Johannes | Art Direction |
| Production | Tom Six | Producer |
| Sound | Patrick Savage | Music |
| Costume & Make-Up | Rachel Elbertse | Makeup Department Head |
| Art | Stefan Thomas | Production Design |
| Art | Caroline Lucciari | Set Decoration |
| Camera | Goof de Koning | Director of Photography |
| Production | Yumi Takada | Casting |
| Sound | Sefi Carmel | Sound Re-Recording Mixer |
| Sound | Eilam Hoffman | Supervising Sound Editor |
| Sound | Tomer Kantor | Sound Editor |
| Sound | Tomer Kantor | Sound Effects Editor |
| Sound | Ariel Levinsohn | Sound Effects Editor |
| Sound | Andrew Lewis | Sound Editor |
