Dr. Lily Houghton enlists the aid of wisecracking skipper Frank Wolff to take her down the Amazon in his dilapidated boat. Together, they search for an ancient tree that holds the power to heal – a discovery that will change the future of medicine.
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| Directing | Jaume Collet-Serra | Director |
| Costume & Make-Up | Paco Delgado | Costume Design |
| Production | John Davis | Producer |
| Production | Hiram Garcia | Producer |
| Art | Jean-Vincent Puzos | Production Design |
| Production | John Fox | Producer |
| Production | Douglas C. Merrifield | Executive Producer |
| Camera | Flavio Martínez Labiano | Director of Photography |
| Production | Dany Garcia | Producer |
| Costume & Make-Up | Stacy Horn | Costume Supervisor |
| Visual Effects | Joe Howes | Visual Effects |
| Crew | Tim McGovern | Digital Effects Supervisor |
| Sound | James Newton Howard | Original Music Composer |
| Editing | Joel Negron | Editor |
| Production | Scott Sheldon | Executive Producer |
| Production | Beau Flynn | Producer |
| Production | Dwayne Johnson | Producer |
| Writing | John Norville | Screenstory |
| Writing | Josh Goldstein | Screenstory |
| Writing | John Requa | Screenstory |
| Writing | Michael Green | Screenplay |
| Writing | Glenn Ficarra | Screenplay |
| Writing | John Requa | Screenplay |
| Art | Chris Hansen | Art Direction |
| Art | Gregory S. Hooper | Art Direction |
| Art | David Lazan | Supervising Art Director |
| Art | Leslie McDonald | Art Direction |
| Art | Drew Monahan | Art Direction |
| Art | Nic Pallace | Art Direction |
| Art | Stella Vaccaro | Art Direction |
| Art | Thomas P. Wilkins | Art Direction |
| Art | Dean Wolcott | Art Direction |
| Art | Larry Dias | Set Decoration |
| Sound | Erik Aadahl | Sound Designer |
| Sound | Erik Aadahl | Supervising Sound Editor |
| Sound | Ignacio Arrojo | ADR Recordist |
| Sound | Ron Bartlett | Sound Re-Recording Mixer |
| Sound | Ian Bender | Boom Operator |
| Sound | Hunter Berk | ADR Mixer |
| Sound | Malte Bieler | Sound Editor |
| Sound | Matt Cavanaugh | Sound Effects Editor |
| Sound | Kevin Cerchiai | Boom Operator |
| Sound | Jack Cucci | Foley Mixer |
| Sound | Joel Erickson | ADR Supervisor |
| Sound | Goeun Lee Everett | Sound Effects Editor |
| Sound | Lee Gilmore | Sound Effects Editor |
| Sound | Robert Jackson | Dialogue Editor |
| Sound | Brandon Jones | Sound Designer |
| Sound | John Marquis | Sound Designer |
| Sound | Victor Mercader | Sound Editor |
| Sound | Dan O'Connell | Foley Artist |
| Sound | Jason Oliver | ADR Mixer |
| Sound | Ralph Osborn | Dialogue Editor |
| Sound | Willard Overstreet | Foley Editor |
| Sound | Aaron Southerland | ADR Mixer |
| Sound | Greg ten Bosch | Sound Effects Editor |
| Sound | Ethan van der Ryn | Supervising Sound Editor |
| Sound | Ethan van der Ryn | Sound Designer |
| Sound | Stuart Welch | ADR Recordist |
| Visual Effects | Jim Berney | Visual Effects Supervisor |
| Visual Effects | Gaia Bussolati | Visual Effects Supervisor |
| Visual Effects | Christopher Downs | Visual Effects Supervisor |
| Visual Effects | John Knoll | Visual Effects Supervisor |
| Visual Effects | Luke Millar | Visual Effects Supervisor |
| Visual Effects | Jake Morrison | Visual Effects Supervisor |
| Visual Effects | Viktor Müller | Visual Effects Supervisor |
| Visual Effects | Stephane Paris | Visual Effects Supervisor |
| Costume & Make-Up | Jordann Aguon | Makeup Artist |
| Costume & Make-Up | Laura Aguon | Makeup Artist |
| Costume & Make-Up | Ozzy Alvarez | Makeup Artist |
| Costume & Make-Up | Merc Arceneaux | Makeup Artist |
| Costume & Make-Up | Rachel Bongard | Makeup Artist |
| Costume & Make-Up | Natalie Bruce | Makeup Artist |
| Costume & Make-Up | Chris Diamantides | Makeup Artist |
| Costume & Make-Up | Addison Foreman | Makeup Artist |
| Costume & Make-Up | André Freitas | Makeup Artist |
| Costume & Make-Up | Derek Garcia | Makeup Artist |
| Costume & Make-Up | Melody Levy | Makeup Artist |
| Costume & Make-Up | Devin Shayla Morales | Makeup Artist |
| Costume & Make-Up | Karen Iboshi Preiser | Makeup Artist |
| Costume & Make-Up | Mark James Ross | Makeup Artist |
| Costume & Make-Up | Aida Scuffle | Makeup Artist |
| Costume & Make-Up | Matt Sprunger | Makeup Artist |
| Costume & Make-Up | Jordan Venetis | Makeup Artist |
| Visual Effects | Michael Furniss | Visual Effects |
| Visual Effects | Kay Hoddy | Rotoscoping Artist |
| Crew | Nicole Vela-Bailey | Stunts |
| Crew | Erika Takacs | Stunts |
| Crew | Lauren Shaw | Stunt Double |
| Crew | Lauren Shaw | Stunt Driver |
| Crew | Lauren Shaw | Utility Stunts |
| Crew | Maya Santandrea | Stunts |
| Crew | Maya Santandrea | Utility Stunts |
| Crew | Alice Rietveld | Stunt Double |
| Crew | Suzanne Reed | Stunts |
| Crew | Allan Poppleton | Stunt Coordinator |
| Crew | Zoey Chang | Stunts |
| Crew | Stacey Carino | Stunts |
| Crew | Bonnie Campanella | Stunts |
| Crew | Hannah Betts | Stunt Double |
| Crew | Hannah Betts | Stunts |
| Crew | Hannah Betts | Utility Stunts |
| Crew | Kelly Bellini | Stunts |
| Crew | Adrienne Ballenger | Stunt Double |
| Crew | Adrienne Ballenger | Stunts |
| Production | Mary Vernieu | Casting Director |
| Production | Marisol Roncali | Casting Director |
| Production | Douglas C. Merrifield | Unit Production Manager |
| Directing | David H. Venghaus Jr. | First Assistant Director |
| Directing | Eric Richard Lasko | Second Assistant Director |
| Visual Effects | Marla Neto Henshaw | Visual Effects Producer |
| Visual Effects | Amber Kirsch | Visual Effects Producer |
| Production | Larry J. Franco | Consulting Producer |
| Production | Petra Holtorf | Co-Producer |
| Production | Lacey Darlene Paulson | Associate Producer |
| Production | David H. Venghaus Jr. | Associate Producer |
| Sound | Jeanette Surga | Supervising Music Editor |
| Visual Effects | Mare McIntosh | Visual Effects Production Manager |
| Visual Effects | Sarah Stauffer | Visual Effects Coordinator |
| Visual Effects | David Gullman | Visual Effects Coordinator |
| Visual Effects | Peter Calvillo | Visual Effects Coordinator |
| Production | Robert Mazaraki | Production Supervisor |
| Art | Margaret Hungerford | Assistant Set Decoration |
| Art | Nathan Krochmal | Assistant Art Director |
| Art | Kristen Nowotarski | Assistant Art Director |
| Costume & Make-Up | Joel Harlow | Makeup Department Head |
| Costume & Make-Up | Adruitha Lee | Hair Department Head |
| Costume & Make-Up | Vanessa Davis | Key Hair Stylist |
| Costume & Make-Up | Kimberly Felix | Key Makeup Artist |
| Directing | Anna Rane | Script Supervisor |
| Sound | Michael B. Koff | Sound Mixer |
| Visual Effects | J.D. Schwalm | Special Effects Supervisor |
| Crew | Eric Cook | Special Effects Coordinator |
| Production | Marc Zakalik | Production Coordinator |
| Crew | Greg Tresan | Animal Wrangler |
| Crew | Carol Tresan | Animal Wrangler |
| Sound | Jim Weidman | Music Editor |
| Sound | David Olson | Music Editor |
| Visual Effects | Paul Ramsden | Animation Supervisor |
| Crew | David Conley | Executive Visual Effects Producer |
| Visual Effects | Andy Taylor | Visual Effects Producer |
| Visual Effects | Reetu Aggarwal | 3D Artist |
| Directing | Alexander Witt | Second Unit Director |
| Crew | John C. Meier | Stunts |
| Crew | Tanoai Reed | Stunt Double |
| Crew | Mike Rufino | Stunts |
| Crew | Shawn Kautz | Stunts |
| Crew | Brian L. Keaulana | Stunts |
| Crew | Thomas Robinson Harper | Stunts |
| Crew | Kris A. Jeffrey | Stunts |
| Crew | Darin Fujimori | Stunts |
| Crew | John Gilbert | Utility Stunts |
| Crew | Kent De Mond | Stunts |
| Crew | Nick Epper | Stunts |
| Crew | Sala Baker | Stunts |
| Crew | Isaac Hughes | Stunts |
| Crew | Anthony N. | Stunts |
| Crew | Dario Perez | Stunts |
| Crew | Nikolas Pelekai | Stunts |
| Crew | Gary Price | Stunts |
| Crew | Remington Steele | Stunts |
| Crew | George Quinones | Stunts |
| Crew | Nick Stanner | Stunts |
| Crew | Steve Upton | Utility Stunts |
| Crew | Robert Tinsley | Stunts |
| Crew | Jason A. Triplett | Stunts |
| Crew | Nico Woulard | Stunts |
| Crew | Marcus Young | Stunts |
| Crew | Thom Williams | Stunts |
| Crew | Tony Vittorioso | Stunts |
| Crew | Tyler Witte | Stunts |
| Production | Riley Flanagan | Key Set Production Assistant |
| Crew | Matt Leonard | Stunts |
| Art | Kevin Houlihan | Set Designer |
